Biomolecular delivery into single cells with high transfection efficiency and cell viability is of great interest for cell-cell communication and cellular therapeutics purpose. In the present study, we have demonstrated the specific single-cell optoporation-based intracellular delivery in monolayer cell culture using PDMS pyramidal Microtips infused with reduced Graphene Oxide(rGO) at the tip. The 2D drawings of microtips were developed using AutoCAD software. The three-dimensional mold for an array of rGO-PDMS pyramidal Microtips device was fabricated using a soft lithographic technique followed by the etching of silicon oxide layer and the silicon layer. The final device was fabricated in PDMS in such a way that rGO was concentrated only at the tips. The scanning electron microscopy(SEM) characterization of the fabricated device was confirmed with the dimensions 106 µm opening width, 75 µm depth, and 100 µm distance between two tips for selective Intracellular delivery. And the dimensions 20 µm opening width, 35.3 µm depth, and 40 µm distance between two tips for uniform Intracellular delivery was also confirmed with SEM characterization. For intracellular delivery studies, the fabricated device was placed on the monolayer cell culture. The biomolecules to be delivered were added to the cell culture medium and exposed to the nanosecond pulse laser. Upon IR laser pulse irradiation, the rGO at the tip of the device generated photothermal cavitation bubbles to disrupt the cell membrane surface and create transient membrane pores to deliver the biomolecules into the cells by a simple diffusion process. Such optoporation-based intracellular delivery occurs only where the PDMS-AuNRs pyramidal microtip is in close proximity to the cell, thus giving the specific, targeted, and single-cell patterned transfection. We have achieved the best results for various biomolecules such as propidium iodide dye, dextran, siRNA, EGFP, and Enzyme in different cell lines (L929, SiHa, N2a) with higher transfection efficiency and cell viability. Such an intracellular delivery approach can be useful in cellular research, therapeutics, and diagnostic applications