Self-excited regenerative vibration or chatter limits the primary requirements like productivity, surface finish and dimensional accuracy of high-speed machining. It is the most critical factor that severely affects the tool-life and life of the machine tool. Out of several methods followed for suppressing and avoiding chatter, machining conditions chosen with stability lobe diagram is the most reliable way. Stability lobe diagrams are typically generated by knowing specific cutting force coefficients and tool point frequency response functions (FRFs). Inaccurate use of tool point FRFs significantly affects the stable regions of stability lobe diagrams. As tool-point FRFs are influenced by gyroscopic effect, centrifugal force, thermal change in bearing dynamics, etc. during machining, it is important to consider the effect of these factors on tool point FRFs in order to generate accurate stability lobe diagrams. This seminar covers a new approach for estimating tool point FRFs during machining operations, employing cutting tool vibration signals. For measuring the vibration at the tip of end mill at different spindle speeds, a non-contact laser vibrometer is used. In order to remove the tooth pass frequency and its harmonics from the measured signals, a comb filter is employed. The filtered signal is subjected to Operational Modal Analysis (OMA) in order to derive tool point FRFs. With the estimated FRFs at different spindle speeds, the stability lobe diagrams are drawn for high-speed milling machine. Comparative study of stability lobe diagrams, drawn with static modal analysis and Operational Modal Analysis, have shown that the cutting conditions chosen from stability lobe diagram derived with OMA, are found to be more realistic for avoiding chatter during high-speed milling applications.