Chikungunya is a mosquito-borne infectious disease, which causes acute fever and joint pain, and can trigger long term arthritis. Development of a fast and simple diagnostic tool for this viral disease is the need of the hour. Biosensors, with their excellent potential for easy, direct and point-of-care applications, are considered to be a promising solution. In this study, we present a label-free detection of chikungunya virus non-structural protein 3 (CHIKV-nsP3), which is responsible for alphavirus replication, using quartz crystal microbalance with dissipation monitoring (QCM-D), and electrochemical impedance spectroscopy (EIS). A labelled sandwich assay using plastic optical fiber biosensors (POF) with gold nanoparticle labels were also performed. The QCM-D and EIS based immunosensors were developed by modifying a gold (Au) electrode surface using a self-assembled monolayer of 11-mercaptoundecanoic acid and 6-mercaptohexanol, and anti-CHIKV-nsP3 antibodies were attached to the surface via carbodiimide coupling reaction. In QCM-D biosensor, the change in frequency of quartz crystal was used to quantify the antigen concentration, and the limit of detection was found to be ~ 446 ng/ml. In EIS immunosensor, the impedance spectra were fit to an equivalent circuit, and the percentage change in charge transfer resistance was correlated to the analyte concentration, and the limit of detection was observed at 8 ng/ml. The limit of detection obtained using the labelled detection of CHIKV-nsP3 using POF was 0.12 ng/ml. With the specificity analysis performed by comparing the responses from pathogenic proteins of other vector-borne diseases, these immunosensors were found to be very specific towards CHIKV-nsP3. The EIS sensor was further tested for matrix effect from human serum, and appreciable recoveries ranging from 97% to 106% was observed. The results illustrate the potential of these immunosensors with CHIKV-nsP3 as a suitable biomarker for the easy, direct and sensitive detection of chikungunya viral disease.