Cellular senescence is a major impediment to cancer development. Current therapies including chemo and radiotherapy, induce senescence in addition to cell death. These senescent cells can be targeted by a novel class of small molecules called senolytic drugs. MicroRNAs are small 20-nucleotide RNA molecules that are dysregulated in cancer and other diseases. One such microRNA, miR-34b, a downstream effector of p53, is dysregulated in multiple cancers including cervical cancer. Ectopic expression of this microRNA has the potential to suppress cell proliferation but the exact mechanism is unknown. We demonstrate that miR-34b suppresses cell proliferation in cervical cancer cells through the induction of cellular senescence and increased oxidative stress. MiR-34b mediates these effects by targeting 3' UTR of TWIST1 as demonstrated by luciferase assay. Targeting TWIST1 using shRNA resulted in the same downstream effects as seen by ectopic miR-34b expression confirming our hypothesis.